kawsar.

Technical University of Denmark

A bioreactor that runs on its own

  • PID control
A bioreactor that runs on its ownTechnical University of Denmark

A PID loop brought the water to about 19.4 °C and held it within ±0.1 °C, with nobody on site.

The work

Keep live mussels in a bioreactor for six days with nobody tending it. The water started at 17.9 °C and had to reach its setpoint and hold there on its own.

Technical University of Denmark · Health Technology · 2025 · Grade 12/12 · Team of 10

What I found

With the water held near 19 °C, the mussels opened their shells as soon as they went in and stayed open for the whole run.

What I did

  1. 01Owned the temperature control, a PID loop on the water.
  2. 02Tuned it from a step-response test: modeled the cooling as a 20 s delay and a 533 s time constant, then set the starting gains with Ziegler–Nichols.
  3. 03Brought it from 17.9 °C to about 19.4 °C and held it there for six days.
  4. 04Led planning for the ten of us.

Results

  • ±0.1 °C typical, ±0.5 °C at worst
  • 6 days unattended
  • 33% less cooling energy with sand as insulation
Two buckets joined by a siphon tube on a lab sink, with a ruler to read the water level

Siphon test: about 20 L/h through two pipes

Two buckets joined by a siphon tube on a lab sink, with a ruler to read the water level
Cutaway render of the 3D-printed OD sensor housing, with the light sensor board under the flow channel

OD sensor design

Cutaway render of the 3D-printed OD sensor housing, with the light sensor board under the flow channel
The breadboard electronics, microcontroller and power supply laid out on a bench

Electronics

The breadboard electronics, microcontroller and power supply laid out on a bench
The full bioreactor on a lab bench: the mussel bucket wrapped in yellow and black bags, a cooler box, breadboard electronics and a laptop

The whole setup

The full bioreactor on a lab bench: the mussel bucket wrapped in yellow and black bags, a cooler box, breadboard electronics and a laptop
A mussel with its shell open inside a green net in the tank

Shells open

A mussel with its shell open inside a green net in the tank
building & testing.
Back to theall works.